How to explain temperature-sensitive mutation,what exactly it is ?

In  temperature sensitive mutation is it lowers the critical melting temperature for the protein, so making it denature at a lower temperature than the normal gene product.Therefore when the temp changes from the 37 to 30 degree there is alterations.These alterations in key portions of a protein may lead to changes in the specificities of the protein.

This is a kind of loss of phenotype phenomena,heat-sensitive mutations, traditionally known as temperature-sensitive (TS) mutations, are powerful tools for studying the functions of all genes.TS mutations are functional at low temperatures, yet nonfunctional at high temperatures, and thus a rise in temperature quickly ablates protein function.

Temperature sensitive mutations are typically missense mutations, which retain the function of a specific essential gene at standard low temperature, lack that function at a defined high temperature, and exhibit partial function at an intermediate temperature

Mutations are ususally found which resulted in a preferential loss of ability to carry out protein synthesis, RNA synthesis, DNA synthesis, cell division, or cell-wall formation.So it depends on the kind of strain you have isolated