Calculate Km and Vmax for phenylalanine-containing peptide is catalyzed by a alpha-chymotrypsin with the following results

The hydrolysis of a phenylalanine-containing peptide is catalyzed by a alpha-chymotrypsin with the following results. Calculate Km and Vmax for the reaction.

Peptide concentration Velocity

2.5 x 10^-4 2.2 x 10 ^ -6

5.0 x 10 ^ -4 5.8 x 10 ^ -6

10.0 x 10 ^ -4 5.9 x 10 ^ -6

15.0 x 10 ^ -4 7.1 x 10 ^ -6

Original data
[S]	[V]
2.5	2.2
5	5.8
10	5.9
15	7.1
1/[S]	1/[V]
0.4	0.4545454545
0.2	0.1724137931
0.1	0.1694915254
0.0666666667	0.14084507

Y=1/vmax =0.0554595078

Vmax=18.0311733672

slope = Km/Vmax=0.9332058421

km=slope*vmax=16.8267963262