| Substrate Value | rate value |
| 1/[S] | 1/[V] |
| 1 | 6993.006993007 |
| 0.25 | 5494.5054945055 |
| 0.167 | 5319.1489361702 |
| 0.125 | 5263.1578947369 |
| 0.083 | 5154.6391752577 |
| 0.067 | 5128.2051282051 |
| 0.05 | 5102.0408163265 |
| So after you plot use the insert tool to fit the curve |
This is the excel file created by me you can use it to calculate km and vmax value



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