Wednesday, 2 April 2014

What is the role of salt during DNA precipitation and extraction?

All the DNA extraction protocols I have seen involve adding salts to the extraction buffer,why is it necessary to add Salt to the DNA for precipitating ,basically the role of salt is to neutralize the charge of the DNA's sugar phosphate backbone. This makes the DNA less hydrophilic (less soluble in water).
Ethanol has a lower dielectric constant than water so it's used to promote ionic bonds between the Na+(from the salt) and the PO3- (from the DNA backbone) causing the DNA to precipitate.

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