Experiment involving membrane proteins are quite messy as storage is a major problem for these kinds of proteins
Membrane proteins and they aren't amenable to freeze thaws even when we add glycerol. The proteins are solubilized in detergent above the cmc so they should be in micelle form in solution. In most case the protein are being held at 4C. Is there any other analytical technique to store these membrane proteins.As freezing would possibly degrade the extracted protein sample.
Membrane proteins and they aren't amenable to freeze thaws even when we add glycerol. The proteins are solubilized in detergent above the cmc so they should be in micelle form in solution. In most case the protein are being held at 4C. Is there any other analytical technique to store these membrane proteins.As freezing would possibly degrade the extracted protein sample.
. One of the ways could be aliquot the samples and snap freeze them in liquid nitrogen and store in -80 degree. if its a membrane protein that are using the cell based system since if it was E. coli then one could have just had your protein expressed through IPTG and just washed the cell pellet in STE buffer and spun it down hard and stored the pellet at -20 degree. The use of Na azide is a effective method too .
