Showing posts with label virus. Show all posts
Showing posts with label virus. Show all posts

Monday, 5 May 2014

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What is the difference between transformation and transfection? How do both of these methods work?

Basically transformation came to be used to describe experiments in which the phenotype of an organism was changed by the uptake of DNA, and because of the way this developed in bacterial systems this DNA was usually a plasmid. Then it became possible to use purified phage DNA whereupon the 'transformed' cells produced phage particles - for obvious reasons this was called transfection.
When efforts turned to getting the same techniques working with cultured animal cells many of the vectors were based on viral genomes (e.g. SV40) and these workers referred to this as transfection. At this point the distinction between the two terms became meaningless, and usage tends to be for historical reasons. Interestingly in molecular manipulations of yeast, where there are no viruses, the transfection word is rarely used.
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Thursday, 13 March 2014

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What types of data convinced researchers that HIV originated when a simian immunodeficiency virus "jumped" to humans?

What types of data convinced researchers that HIV originated when a simian immunodeficiency virus "jumped" to humans?

 ancestors

as well as humans

plausible mechanisms for HIVs to be transmitted to monkeys or chimps

SIVs

mechanisms for SIVs to be transmitted to humans through butchering or contact with pets

HIVs


ans---

 HIVs can infect primates as well as humans. Researchers estimate that sometime in the 1930s a form of simian immunodeficiency virus, SIV, jumped to humans in central Africa. The mutated virus became the first human immunodeficiency virus.

mechanisms for SIVs to be transmitted to humans through butchering or contact with pets

6) SIVs are ancestral to the HIV.

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